Homogenate-Ultrasonic Synergistic Extraction of Genistin and Sophoricoside from Styphnolobium japonicum Pods

Using the Styphnolobium japonicum pods as raw materials and ethanol as extraction solvent, genistin and sophoricoside were extracted by homogenate-ultrasonic synergistic extraction method. Effects of ethanol volume fraction, liquid-solid ratio, homogenate time, homogenate speed, ultrasonic time, ultrasonic power, cavitation time and buffer time on the yield of genistin and sophoricoside were investigated by the quantitative method of high performance liquid chromatography. The best extraction conditions were as follows: ethanol volume fraction of 50%, liquid-solid ratio of 20 mL·g⁻¹, homogenate time of 3 min, homogenate speed of 22000 r·min⁻¹, ultrasonic time of 35 min , ultrasonic power of 350 W, cavitation time of 2.5 s and buffer time of 1.5 s. Compared with the traditional extraction method, the homogenate ultrasonic-synergistic extraction method had the advantages of high efficiency, environmental protection and energy saving.