Interannual Variation of Bacteria Communities in Red Pandas Foraging Bamboos Phyllosphere

As an important indicator organ of bamboo forest health, bamboo leaves are an important place for the survival and reproduction of microorganisms around bamboo leaves, as well as an important entrance for pathogenic microorganisms to invade, which will affect the health of wild red pandas. In order to investigate the interannual variation of bacteria communities in red pandas foraging bamboos phyllosphere, high-throughput sequencing was used to analyze the composition and diversity of phyllosphere bacteria of Yushania ailuropodina in Sichuan Meigu Dafengding National Nature Reserve. The results showed that OTUs was most abundant in November, and the number of OTUs decreased from September 2021 to August 2022. Proteobacteria, Bacteroidota and Acidobacteriota were the predominant phyla in whole 12 months, and the relative abundance of Proteobacteria in June, July and August was higher than that in other months. But the relative abundance of Bacteroidota was lower than that of other months. The relative abundance of Pseudomonas in June, July and August was significantly higher than that in other months, but the relative abundance of Pseudomonas and 1174-901-12 in June, July and August was lower than that in other months. The Sobs and Shannon index of phyllosphere bacteria in September, October and November were higher than those in other months. PCoA analysis based on weighted/unweighted UniFrac distance showed that the bacterial community structure of phyllosphere bacteria varied from different months at OTU level. This study improved our understanding of phyllosphere bacteria in red pandas foraging bamboos, and provided a reference for the protection of bamboo resources.