Study on Callus Induction and Differentiation of Gerbera delavayi Leaves

Using Gerbera delavayi leaves as explants, the methods of callus induction and differentiation were studied. The results showed that the green callus with a diameter 0.5 cm, compact structure and hard texture could be induced from the sterile leaves of Gerbera delavayi in the medium of WPM+ 0.6 mg·L⁻¹6-BA + 0.45 mg·L⁻¹ NAA + 30 g·L⁻¹ sucrose. The calli differentiated into adventitious buds after 30 days in the medium of WPM+ 0.75 mg·L⁻¹6-BA+ 0.15 mg·L⁻¹ NAA+30 g·L⁻¹ sucrose. After the shoots were transferred to 1/2DKW+1.0 mg·L⁻¹IBA+0.1 mg·L⁻¹ NAA+30 g·L⁻¹ sucrose for 30 days, 5-13 adventitious roots with root hairs were developed.