Study on Soluble Sugar Content in Tissue Culture Explants of Tripterygium wilfordii
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Graphical Abstract
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Abstract
In order to explore the effect of soluble sugar content in tissue culture of Tripterygium wilfordii Hook. f, anthraquinone ketene method was used to measure the soluble sugar content at different stages of tissue culture of Tripterygium wilfordii. The results showed that 14 d was a short period for the growth and development of Tripterygium wilfordii explants, and sucrose was the best carbon source in the process of root culture stage. The optimal concentration was 30 g·L−1 and the rooting rate reached 100%. The content of soluble sugar increased at 0−7 d and 14−21 d, but decreased at other time periods, which indicated that soluble sugar was an important energy substance. Among different carbon source treatments, sucrose was the best carbon source. Glucose not only caused poor rooting effect of Tripterygium wilfordii explants, but also inhibit the growth and development, which made the explants in a nutrient deficiency state. In the process of tissue culture of Tripterygium wilfordii, exogenous sucrose could be added to promote the growth and development of Tripterygium wilfordii explants.
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