Study on Callus Induction and Regeneration of Anthurium andraeanum Tiller Bud

Using anthurium andraeanum tiller bud as explant, the methods of callus induction and regeneration at bud base were studied. The results showed that the sterile tiller seedlings could induce adamant callus with a diameter of 0.5—1.0 cm and hard texture in the medium of MS+0.5 mg/L 6-BA+0.05 mg/L NAA+30 g/L sucrose. The callus differentiated into buds in the medium of 1/2MS+0.2 mg/L 6-BA+0.05 mg/L NAA+ 30 g/L sucrose. On average, each callus had 6 effective buds. When the buds were transferred to the medium of 1/2MS+0.2—0.5 mg/L IBA+20 g/L sucrose, they could develop into rooting seedlings with a height of 3 cm or more, with adventitious roots and aerial roots.