Abstract: In this study,Populus schneideri var. tibetica's stems with buds were selected as explants. A series of tests were conducted on disinfection and sterilization,the induction of adventitious buds,the proliferation of regeneration buds,strong regenerated seedlings,rooting and transplantation in order to study the best training methods during the tissue culture process,and to screen out medium components for plant differentiation and rooting. The results showed that 45 seconds of alcohol disinfection and 8 minutes of mercuric chloride sterilization worked best. The bud induction rate of explants reached 79% in MS + NAA 0.05 + 6-BA 2.0 medium,and the axillary bud sprouted after 10 days. The proliferation coefficient was up to 5.8 in MS + NAA 0.1 + 6-BA 2.0 medium,displaying the best effects. The effect of promoting rooting was significant in 1/2 MS + NAA 0.5 medium after strengthening the regenerated seedlings in MS + NAA 0.1 + 6-BA 0.5 medium. This would provide technical reserve for the cultivation speed acceleration and genetic improvement of Populus schneideri var. tibetica.