Abstract: The high-efficient cross-infection seedling-raising technique of truffles were proposed firstly in this papor. The hazel and pine seeds were inoculated in the tub containing the water-retaining agent adsorbing the suspension of truffle spore. After five months, the mycorrihzal seedlings of truffles were transplanted. 3 000 mycorrhizal seedlings were produced by using only 200g of truffle ascocarp. This technique would be very useful in the cultivation of mycorrihzal seedlings.