川渝地区三年桐核心种质的初步构建

Establishment of Key Germ Plasm of Vernicia fordii in Sichuan and Chongqing Areas

摘要: 对川渝地区300份三年桐资源按照种群的方式分成12组,然后运用分组聚类法分别按30%、25%、20%、15%、10%的比例抽取5个候选核心种质,比较Shannon表型遗传多样性指数HI并与总体种质进行差异显著性检验,结果显示20%为最佳取样比例,从而确定核心种质60份。通过对核心种质进行符合性检验,平均值符合率、标准差平均符合率和变异系数平均符合率分别为98.07%、94.12%和95.70%。结果表明,核心种质基本上可以较好的代表总体种质的遗传变异。

Abstract: The 300 parts of VF germplasm materials in Sichuan and Chongqing areas were divided into 12 groups according to the population way, then by group clustering method, 5 candidates were collected (in the proportion of 30%, 25%, 20%, 15% and 10%) and their genetic diversity indexes were compared and the significance of their difference was tested. Finally 60 parts of key germ plasm were established under 20% of the sampling rate. Through testing the characteristic value coincidence of key germ plasma, the mean compliance rate, the standard deviation rate and the coefficient of variation were 98.07%, 94.12% and 95.70%, which showed that the primary key germ plasm was able to represent genetic variation of the total germplasm.