西康花楸种胚快繁技术研究

Study on rapid propagation technology of Sorbus prattii

摘要: 以西康花楸种胚为外植体，研究不同激素和基础培养基类型对西康花楸种胚萌发、增殖、芽苗生根的影响。结果表明，西康花楸种胚在MS+0.5 mg·L⁻¹6-BA培养基上平均萌发率为90.67%；芽苗转入MS+0.25 mg·L⁻¹ 6-BA+0.025 mg·L⁻¹ NAA+30 g·L⁻¹ 蔗糖+7 g·L⁻¹ 卡拉胶的培养基，平均增殖倍数3.14，持续增殖时，基础培养基改为DKW可持续培育健壮芽苗；芽苗在DKW+0.02 mg·L⁻¹ IBA+30 g·L⁻¹ 蔗糖+7 g·L⁻¹ 卡拉胶的生根培养基中平均生根率92.5%；生根苗经炼苗后移栽，冬季存活率达90%以上。

Abstract: Using the embryo of Sorbus prattii as the explants, the effects of different hormones and basal medium types on the germination, proliferation and rooting were studied. The results showed that the average germination rate of Sorbus prattii seed embryos on MS+0.5 mg·L⁻¹ 6-BA medium was 90.67%. The sprouts were transferred to MS+0.25 mg·L⁻¹ 6-BA+0.025 mg·L⁻¹ NAA+30 g·L⁻¹ sucrose +7 g·L⁻¹ carrageenan, with an average multiplication factor of 3.14. When the proliferation continues, the basic medium was changed to DKW for sustainable cultivation of robust buds. The averge rooting rate of sprouts in DKW+0.02mg·L⁻¹ IBA+30 g·L⁻¹ sucrose +7 g·L⁻¹ carrageenan was 92.5%. The rooted seedlings were transplanted after hardening, and the survival rate in winter was over 90%.