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不同种源银杏同质园幼苗生长与叶片药用成分含量比较

Variations of Seedling Growth and Medicinal Components Contents in Leaves of Ginkgo biloba from Different Provenances in Common Garden

  • 摘要: 开展不同种源银杏同质园试验,揭示环境对不同种源银杏的影响,为选育药用价值高的银杏种源和品种提供依据。收集了川内4个不同种源的银杏种子,经同质园栽培后,对其幼苗生长和叶片药用成分含量进行测定分析,并对各测定结果进行相关性分析。结果显示:(1)各种源间种子横径、纵径和百粒重均极显著差异(P<0.01),且均开江最大;(2)各种源间苗高、单株叶数、单叶重和单株叶重均极显著差异(P<0.01),冠幅显著差异(P=0.02)。其中苗高、单株叶数、单株叶重、冠幅均开江最大,单叶重泸定最大;(3)各种源间叶片萜类内酯、白果内酯含量呈极显著差异(P<0.01),黄酮、银杏内酯A和银杏内酯B含量均显著差异(P=0.02,P=0.03,P=0.01)。其中黄酮含量苍溪最高,萜类内酯、白果内酯、银杏内酯A、银杏内酯B含量均泸定最高;(4)苗高与单株叶重呈极显著正相关(r=0.912);萜类内酯与白果内酯和银杏内酯B含量(r=0.928和0.962)均呈极显著正相关。不同种源的银杏同质园栽培结果表明,由于亲代长期受种源地环境的影响,子代幼苗的生长以及药用成分含量具有显著差异。

     

    Abstract: The Common garden experiment of Ginkgo biloba from different provenances was carried out to reveal the influence of environment on different provenances of Ginkgo biloba, so as to provide basis for breeding the provenances and varieties of Ginkgo biloba with high medicinal value. In this study, four ginkgo seeds from different provenances in Sichuan were collected, and after being cultivated in common garden, the seedling growth and the content of medicinal components in leaves were measured and analyzed, and the correlation analysis of the results was carried out. The results were as follows: (1) There were significant differences in the transverse diameter, vertical diameter and 100-seed weight from different provenance (P<0.01), and Kaijiang were the largest. (2) There were significant differences in seedling height, leaf number per plant, individual leaf weight and leaf weight per plant from different provenances (P<0.01), and significant differences in crown width (P=0.02). Among them, seedling height, leaf number per plant, leaf weight per plant and crown width are the largest, and single leaf weight is the largest. (3) There were significant differences (P<0.01) in the contents of terpene lactones and ginkgolides in leaves from various sources, and significant differences in the contents of flavonoids, Ginkgolides A and Ginkgolides B (P=0.02, P=0.03, P=0.01). Among them, the content of flavonoids is the highest in Cangxi, and the contents of terpenoids, ginkgolides, Ginkgolides A and Ginkgolides B are the highest in Luding. (4) Seedling height was positively correlated with the individual leaf weight (r=0.912). Terpene lactones were positively correlated with the content of bilobalide and Ginkgolide B (r=0.928, 0.962). The results showed that Ginkgo biloba from different provenances had significant differences in the growth of offspring seedlings and the content of medicinal components after cultivation in common garden, which was due to the long-term influence of parents of the environment of the provenance.

     

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