Abstract: In order to select excellent poplar clones in the mid-altitude mountain area in Southwest Sichuan, further meeting the needs of local afforestation and speeding up the development of poplar industry, 28 poplar clones were introduced respectively, and Butuo county was selected to carry out the seedling test. Using ground diameter and seedling height as indexes, excellent clones suitable for growing in this area were preliminarily selected. By using the methods of variance analysis and multiple comparison analysis, the results showed that there were great differences in seedling growth traits among 1-year-old poplar clones, and Nanlin 312 was the best at seedling stage according to ground diameter and seedling height, which provided a reference for the popularization and application of excellent poplar clones in the mid-altitude mountain area s in Southwest Sichuan.