Abstract: According to the technical problems of long seedling growth cycle, low seedling emergence rate and irregular seedling emergence of Paris polyphylla, the effects of different germination methods, seedling growing substrate and seed size on seed germination rate were explored, which could provide reference for rapid seedling growth of Paris polyphylla.The results showed that the germination rate and germination time were significantly affected by germination accelerating method and seedling growing substrate, but the germination time was not significantly affected by seed size. Among all the treatment, the seeds treated by double-low and double-high alternate temperature method were sown with humus soil, coconut brick and sand mixed substrate, which had the best seedling raising effect, with shortened germination time and improved germination rate. The germination time was only about 120 days, the germination rate was as high as 82%, the emergence time was short, the seedling rate was high, and the emergence was neat.