Abstract: In view of the technical problems such as long seedling breeding cycle, low seedling emergence rate and irregular seedling emergence of Euscaphis japonica seed, effects of different ways of accelerating germination and storage media on seed germination rate were explored in order to provide reference for efficient seedling breeding of Euscaphis japonica. The results showed that the most suitable seed germination treatment was soaking at 50 ℃, soaking time was 72 hours and GA3 concentration was 500 mg/L. The most suitable storage conditions were air-dried in natural environment.