Abstract: The wild macrofungi of Wanglang National Nature Reserve was investigated, classified and identified, which provided a reliable basis for the development and utilization of fungi resources. According to preliminary investigation, there were 218 species of macrofungi, belonging to 2 subphyla, 5 classes and 10 orders, 37 families, 92 genuses. From the ecological distribution, macrofungi were mainly distributed in coniferous forest, followed by coniferous and broad-leaved mixed forest and broad-leaved forest. From the economic value, there were 111 kinds of edible fungi species, 31 kinds of medicinal fungi species, 58 kinds of fungi species with anticancer active substances and 41 kinds of poisonous fungi species.