WE ARE COMMITTED TO REPORTING THE LATEST FORESTRY ACADEMIC ACHIEVEMENTS

Volume 43 Issue 1
Mar.  2022
Article Contents
Turn off MathJax

DENG J, WANG G, YAO C, et al. Effect of light quality on morphological and physiological indexes of Dendrobium denneanum[J]. Journal of Sichuan Forestry Science and Technology, 2022, 43(1): 19−23 doi: 10.12172/202103300001
Citation: DENG J, WANG G, YAO C, et al. Effect of light quality on morphological and physiological indexes of Dendrobium denneanum[J]. Journal of Sichuan Forestry Science and Technology, 2022, 43(1): 19−23 doi: 10.12172/202103300001

Effect of Light Quality on Morphological and Physiological Indexes of Dendrobium denneanum


doi: 10.12172/202103300001
More Information
  • Received Date: 2021-03-30
    Available Online: 2022-01-13
  • Publish Date: 2022-03-02
  • In order to find a new method to improve the ornamental value and economic value of Dendrobium denneanum, Dendrobium denneanum was used as the experimental material to study the effect of light quality on its morphological and physiological indexes. Red and blue light was used in the experiment. The treatment groups were T1 group (red: blue = 3∶1), T2 group (red: blue = 1∶1), T3 group (red: blue = 1∶3), and the control group was white light. All the treatments were irradiated for 2 hours every day, and samples were taken for detection at 7, 14, 21 and 28 days. The results showed out: (1) T1 light quality (red light: blue light = 3∶1) could effectively increase the stem length of Dendrobium denneanum after 7 days of irradiation, and could promote the stem diameter after 21 days of irradiation; (2) T2 light quality (red light: blue light = 1∶1) could promote the stem length after 7 days of irradiation; (3) T3 light quality (red light: blue light = 1∶3) could effectively prolong the flowering period of Dendrobium denneanum, and could effectively increase the content of chlorophyll a and b at day 7th, while could effectively increase the content of soluble protein at day 21. The results indicated that different ratios of red and blue light irradiation could effectively regulate the flowering period and growth of Dendrobium denneanum and increase its ornamental value.
  • 加载中
  • [1] 张海波,张国禹,黄桂云等. 蝴蝶兰的组织培养研究[J]. 中国科技纵横,2005(16):238−239.
    [2] 赖永旭. 从药用石斛研究进展看生药学的发展趋势[J]. 中国实用医药, 2008, 3(14).
    [3] 庞敏霞,戚勇,陈丁江,姚国祥. 铁皮石斛品质性状影响因子的研究进展[J]. 浙江农业科学,2020,61(03):502−508.
    [4] 李茹,李枝林,商正蕊,等. 不同LED光质对铁皮石斛瓶内开花的影响[J]. 南方农业学报,2019,50(07):1550−1556. doi: 10.3969/j.issn.2095-1191.2019.07.20
    [5] 周鹏,张敏,吴双竹,等. 不同红蓝光质比LED光源处理对铁皮石斛试管苗移栽的影响[J]. 江苏林业科技,2017,44(03):19−22. doi: 10.3969/j.issn.1001-7380.2017.03.005
    [6] 郑胤建,汪玉洁,陈田甜,等. 光质与基质互作对铁皮石斛生长的影响[J]. 农业工程技术,2016,36(1):54−58.
    [7] Yuan Lin, Jia Li; Bo Li, Tao He, et al. Effects of light quality on growth and development of protocorm-like bodies of Dendrobium officinale in vitro[J]. Plant Cell, Tissue and Organ CultureVolume 105, Issue 3. 2011. PP 329−335.
    [8] 尚文倩,王政,侯甲男,郑卫杰,何松林. 不同红蓝光质比LED光源对铁皮石斛试管苗生长的影响[J]. 西北农林科技大学学报(自然科学版),2013,41(05):155−159.
    [9] 李婷婷, 淳泽, 罗傲雪, 等. 不同LED光质对叠鞘石斛生理及品质的影响[N]. 应用与环境生物学报, 2017, 23(5): 934−938.
    [10] Zhao H P, Sun X, Xue M, et al. Antioxidant enzyme responses induced by whiteflies in tobacco plants in defense againstaphids: catalase may play a dominant role[J]. Plos One, 2016, 11(10): e0165454. doi: 10.1371/journal.pone.0165454
    [11] Walker J R L. Spectrophotometric determination of enzymeactivity: alcohol dehydrogenase(ADH)[J]. Biochemistrand Molecular Biology Education, 1992, 20(1): 42−43.
    [12] 关玲,赵密珍,于红梅. 套作玉米对草莓生长生理特性及其产量和品质的影响[J]. 西北植物学报,2018,38(9):1692−1699.
    [13] 娄钰姣. 光质对铁皮石斛生长及次生代谢产物的积累调控[D]. 成都: 四川农业大学, 2016.
    [14] 刘慧雯. LED光质对铁皮石斛组培拟原球茎和幼苗生长及主要有效成分的影响[D]. 山东农业大学, 2017.
    [15] 林小苹,赖钟雄. 不同光质条件下铁皮石斛多糖含量与磷酸烯醇式丙酮酸羧化酶基因表达变化[J]. 热带作物学报,2017,38(05):838−842. doi: 10.3969/j.issn.1000-2561.2017.05.009
    [16] 闻婧,李倩中,李淑顺,荣立苹,唐玲,黎瑞. 不同LED光源对铁皮石斛生长和叶绿素荧光参数的影响[J]. 江苏农业学报,2016,32(04):910−916. doi: 10.3969/j.issn.1000-4440.2016.04.031
    [17] 林小苹,赖钟雄. 不同光质对铁皮石斛光合特性和多糖积累的影响[J]. 北方园艺,2016(13):154−158.
    [18] 彭金彬. 光质对金钗石斛生长及品质的影响[J]. 福建林业科技,2015,42(04):67−71.
    [19] 彭金彬. 不同光质比对金钗石斛生长及有效成分的影响[J]. 河北林业科技,2015(01):9−13. doi: 10.3969/j.issn.1002-3356.2015.01.004
    [20] 孙启文. LED不同光质对紫皮石斛组培苗光合特性及生长的影响[J]. 现代农业,2013(07):10−12. doi: 10.3969/j.issn.1008-0708.2013.07.007
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Figures(1)  / Tables(7)

Article views(321) PDF downloads(15) Cited by()

Related
Proportional views

Effect of Light Quality on Morphological and Physiological Indexes of Dendrobium denneanum

doi: 10.12172/202103300001
  • 1. College of Landscape Architecture, Sichuan Agricultural University, Chengdu 610030, China
  • 2. Collge of Forestry, Sichuan Agricultural University, Chengdu 610030

Abstract: In order to find a new method to improve the ornamental value and economic value of Dendrobium denneanum, Dendrobium denneanum was used as the experimental material to study the effect of light quality on its morphological and physiological indexes. Red and blue light was used in the experiment. The treatment groups were T1 group (red: blue = 3∶1), T2 group (red: blue = 1∶1), T3 group (red: blue = 1∶3), and the control group was white light. All the treatments were irradiated for 2 hours every day, and samples were taken for detection at 7, 14, 21 and 28 days. The results showed out: (1) T1 light quality (red light: blue light = 3∶1) could effectively increase the stem length of Dendrobium denneanum after 7 days of irradiation, and could promote the stem diameter after 21 days of irradiation; (2) T2 light quality (red light: blue light = 1∶1) could promote the stem length after 7 days of irradiation; (3) T3 light quality (red light: blue light = 1∶3) could effectively prolong the flowering period of Dendrobium denneanum, and could effectively increase the content of chlorophyll a and b at day 7th, while could effectively increase the content of soluble protein at day 21. The results indicated that different ratios of red and blue light irradiation could effectively regulate the flowering period and growth of Dendrobium denneanum and increase its ornamental value.

  • 叠鞘石斛(Dendrobium denneanum)属于兰科石斛属植物,是线叶石斛的变种,为多年生附生草本植物,花朵色泽艳丽,气味芳香,极具观赏性,为园林观赏主要花卉之一,与文心兰、卡特兰、蝴蝶兰合称“四大观赏洋花” [1] 。石斛属植物除观赏价值外,还是常用中药材,被民间称为“仙草”。自上世纪70年代以来,石斛的需求量大增,野生石斛资源已经不能满足人们的需求[2]。近年来,石斛的来源主要为人工栽培,而人工栽培时温度、光照、激素及栽培措施等对石斛的品质影响非常大。其中光照可通过光照强度、光周期、光质来影响植物生长。现有研究发现光质会影响铁皮石斛的生长及开花[3-4]。然而,目前仍无研究揭示光质对叠鞘石斛的影响。因此,我们拟采用不同组合的红蓝光考察光质对叠鞘石斛开花时间、花朵形态、花期长短、石斛生长状态、生理变化的影响。从而指导光质在叠鞘石斛的人工栽培上如何提高其观赏性和经济价值。

    • 供试材料为叠鞘石斛,种植于四川农业大学成都校区(东经103°86'、北纬30°71')第五教学楼七楼光照架上,光照架遮光,置于通风处。

    • 实验采用LED光源,考察不同组合的红蓝光对叠鞘石斛花期、性状、光合作用的影响。在保证其他环境条件(温度、湿度)相同的情况下,参考光质对石斛的相关研究[5-9],本实验选择三种不同组合比例的红蓝光质,即T1组(红光:蓝光=3∶1)、T2组(红光:蓝光=1∶1)、T3组(红光:蓝光=1∶3),对照组采用白光照射。每间隔7 d取一次样,检测时间共28 d。

      叠鞘石斛种植于花盆中自然生长,每个光质处理组种植4盆,每盆种植30株石斛,每周进行施肥等常规养护工作,定期除杂草。每隔7 d,用2%的过磷酸钙或1%的硫酸钾进行根外施肥,2~3 d浇一次水,以培养基质被浸透为准。

    • 由于叠鞘石斛的生长状况和花期长短直接影响到人们的观赏体验,故实验对叠鞘石斛的茎粗、茎长、花径、花期进行了测定,同时测定了叶绿素a含量、叶绿素b含量、类胡萝卜素含量、可溶性糖浓度、MDA浓度、可溶性蛋白质含量、脯氨酸含量[10-12],从而探究不同处理对叠鞘石斛生长的影响。

    • 利用Microsoft Office Excel 统计软件与IBM SPSS Statistics 24进行数据分析。

    2.   结果与分析
    • 在光照处理过程中,叠鞘石斛陆续开花,我们测试了不同花期时的花朵的直径、植株茎长与茎粗,记录了各组光质处理组的花期。

    • 表1-表3可知,在现蕾期,采用T2(红光:蓝光=1∶1)光质照射时,有利于增大的花径;T1(红光:蓝光=3∶1)和T2(红光:蓝光=1∶1)光质在照射7 d时,均能显著增加茎长,但照射天数延长后效果不明显;T1和T2组在照射天数较长(大于21 d)时可以显著增加茎粗,其中红光:蓝光=3∶1对茎粗的影响更大。

      花期 Flowering stageT1光质 T1 light qualityT2光质 T2 light qualityT3光质 T3 light qualityCK对照 CK
      现蕾期 Budding stage0.83 ± 0.05 bc1.46 ± 0.11 a1.00 ± 0.20 b0.70 ± 0.10 c
      始花期 Mid-bloom stage3.26 ± 0.25 a2.60 ± 0.40 b2.86 ± 0.41 ab3.23 ± 0.05 a
      盛花期 Full-bloom stage4.16 ± 0.35 a3.80 ± 0.43 a4.13 ± 0.23 a4.13 ± 0.37 a
      衰败期 Decline stage1.93 ± 0.30 a1.53 ± 0.05 b1.93 ± 0.20 a1.60 ± 0.10 ab
        h:小时 T1:红光:蓝光=3∶1 T2:红光:蓝光=1∶1 T3:红光:蓝光=1∶3 CK:白光对照。
        Note: h: hour; T1: red light: blue light =3∶1; T2: red light: blue light =1∶1; T3: red light: blue light =1∶3; CK: white light.

      Table 1.  Effect of light quality on flower diameter of Dendrobium denneanum

      照射天数(d) Irradiation daysT1光质 T1 light qualityT2光质 T2 light qualityT3光质 T3 light qualityCK对照 CK
      733.23 ± 8.32 a33.23 ± 1.95 a28.46 ± 2.21 a29.83 ± 0.92 a
      1433.36 ± 8.10 a33.36 ± 1.72 a29.60 ± 2.95 a31.50 ± 1.37 a
      2134.03 ± 7.56 a34.60 ± 1.64 a33.86 ± 3.28 a32.03 ± 0.47 a
      2834.03 ± 7.56 a35.26 ± 1.36 a35.36 ± 2.04 a32.53 ± 0.30 a
        d:天数 h:小时 T1:红光:蓝光=3∶1 T2:红光:蓝光=1∶1 T3:红光:蓝光=1∶3 CK:白光对照。
        Note: d: Day; h: hour; T1: red light: blue light =3∶1; T2: red light: blue light =1∶1; T3: red light: blue light =1∶3; CK: white light.

      Table 2.  Effect of light quality on stem length of Dendrobium denneanum

      照射天数(d) Irradiation daysT1光质 T1 light qualityT2光质 T2 light qualityT3光质 T3 light qualityCK对照 CK
      70.46 ± 0.11 a0.48 ± 0.07 a0.38 ± 0.02 a0.43 ± 0.02 a
      140.46 ± 0.11 a0.48 ± 0.05 a0.41 ± 0.02 a0.43 ± 0.02 a
      210.46 ± 0.11 a0.48 ± 0.05 a0.45 ± 0.05 a0.45 ± 0.05 a
      280.53 ± 0.05 a0.50 ± 0.05 a0.46 ± 0.05 a0.45 ± 0.05 a
        d:天数 h:小时 T1:红光:蓝光=3∶1 T2:红光:蓝光=1∶1 T3:红光:蓝光=1∶3 CK:白光对照。
        Note: d: Day; h: hour; T1: red light: blue light =3∶1; T2: red light: blue light =1∶1; T3: red light: blue light =1∶3; CK: white light.

      Table 3.  Effect of light quality on stem diameter of Dendrobium denneanum

    • 表4可以看出,经红蓝光照射后各处理组的叠鞘石斛的花期均长于对照组。在三种光质处理组中,花期最长的是T3光质处理组,与对照相比,其花期延长了33.25%,且与对照组相比,差异不显著。表明光质为T3(红光:蓝光=1∶3)时会显著延长叠鞘石斛的花期,有利于增加叠鞘石斛的观赏价值。

      T1光质 T1 light qualityT2光质 T2 light qualityT3光质 T3 light qualityCK对照 CK
      9.33 ± 2.51 a10.00 ± 1.00 a10.66 ± 2.08 a8.00 ± 0.05 a
        T1:红光:蓝光=3∶1 T2:红光:蓝光=1∶1 T3:红光:蓝光=1∶3 CK:白光对照。
        Note: T1: red light: blue light =3∶1; T2: red light: blue light =1∶1; T3: red light: blue light =1∶3; CK: white light.

      Table 4.  Effect of light quality on flowering stage of Dendrobium denneanum

      由此可见,T2光质可增加现蕾期花径,T3光质可延长花期。

    • 表567可知, T1、T3光质在照射7天时,能显著增加叶绿素a的含量;T3光质组照射7天时,能显著增加叶绿素b含量;而光质的照射对类胡萝卜素含量没有明显影响。

      照射天数(d) Irradiation daysT1光质 T1 light qualityT2光质 T2 light qualityT3光质 T3 light qualityCK对照 CK
      70.74 ± 0.17 ab0.48 ± 0.07 bc0.83 ± 0.16 a0.45 ± 0.12 c
      140.54 ± 0.03 b0.69 ± 0.07 a0.70 ± 0.04 a0.59 ± 0.04 b
      210.84 ± 0.02 a0.74 ± 0.04 b0.85 ± 0.02 a0.82 ± 0.01 a
      280.81 ± 0.01 a0.83 ± 0.01 a0.56 ± 0.03 b0.57 ± 0.10 b
        d:天数 h:小时 T1:红光:蓝光=3∶1 T2:红光:蓝光=1∶1 T3:红光:蓝光=1∶3 CK:白光对照
        Note: d: Day; h: hour; T1: red light: blue light =3∶1; T2: red light: blue light =1∶1; T3: red light: blue light =1∶3; CK: white light.

      Table 5.  Effect of light quality on chlorophyll a content of Dendrobium denneanum

      照射天数(d) Irradiation daysT1光质 T1 light qualityT2光质 T2 light qualityT3光质 T3 light qualityCK对照 CK
      70.34 ± 0.08 a0.32 ± 0.01 a0.39 ± 0.15 a0.26 ± 0.13 a
      140.18 ± 0.03 b0.20 ± 0.03 ab0.29 ± 0.08 a0.25 ± 0.03 ab
      210.34 ± 0.04 a0.22 ± 0.0 a0.24 ± 0.10 a0.35 ± 0.00 a
      280.38 ± 0.06 a0.35 ± 0.05 b0.35 ± 0.02 ab0.37 ± 0.09 ab
        d:天数 h:小时 T1:红光:蓝光=3∶1 T2:红光:蓝光=1∶1 T3:红光:蓝光=1∶3 CK:白光对照
        Note: d: Day; h: hour; T1: red light: blue light =3∶1; T2: red light: blue light =1∶1; T3: red light: blue light =1∶3; CK: white light.

      Table 6.  Effect of light quality on chlorophyll b content of Dendrobium denneanum

      照射天数(d) Irradiation daysT1光质 T1 light qualityT2光质 T2 light qualityT3光质 T3 light qualityCK对照 CK
      70.14 ± 0.02 a0.10 ± 0.01 a0.16 ± 0.01 a0.11 ± 0.05 a
      140.08 ± 0.00 b0.15 ± 0.01 a0.12 ± 0.03 ab0.11 ± 0.02 ab
      210.10 ± 0.02 b0.12 ± 0.02 ab0.16 ± 0.01 a0.15 ± 0.00 a
      280.09 ± 0.03 a0.14 ± 0.07 a0.05 ± 0.02 a0.09 ± 0.02 a
        d:天数 h:小时 T1:红光:蓝光=3∶1 T2:红光:蓝光=1∶1 T3:红光:蓝光=1∶3 CK:白光对照
        Note: d: Day; h: hour; T1: red light: blue light =3∶1; T2: red light: blue light =1∶1; T3: red light: blue light =1∶3; CK: white light.

      Table 7.  Effect of light quality on carotenoid content of Dendrobium denneanum

    • 图1可知, T1光质组照射7d时,T2光质组照射21d时能明显增加可溶性糖含量;T1光质照射14d时,T3光质照射21d时能明显增加可溶性蛋白质含量;T1、T3光质能明显减少植株的氧化进程,从而降低植株的脯氨酸含量;T2光质能显著降低MDA浓度,且效果最好,其次为T3光质处理组,而T1光质照射天数不超过14d时植株MDA浓度极显著大于对照组,说明短时间的T1光质使得T1植株防御系统受到影响,加剧植株氧化进程,从而导致MDA含量增加。

      Figure 1.  Effect of light quality on the contents of soluble sugar, soluble protein, proline and MDA in Dendrobium denneanum

    3.   结论
    • 不同光质是影响叠鞘石斛生长发育和有效物质积累的直接因素,不同光质组合对石斛的各形态和生理指标的影响也不相同[4-7, 13],光质是调控石斛生长发育与开花的重要手段[14-16],也是调控石斛各种次生代谢产物含量的重要环境因子[17-20]

    • 叠鞘石斛在接受不同的光质处理之后,其花径大小与对照组相比,在各个阶段均有增加,但仅T2(红光:蓝光=3∶1)光质处理后的花径在现蕾期与对照相比有显著性差异。T3(红光:蓝光=1∶3)光质可显著延长花期,增加叠鞘石斛的观赏价值。T1(红光:蓝光=3∶1)光质在短期照射时,可显著增加叠鞘石斛的茎粗,长期照射时可显著增加其茎长,从而增加叠鞘石斛的生物量,有利于增加观赏性。

    • T1(红光:蓝光=3∶1)、T3(红光:蓝光=1∶3)光质在照射7 d时,能显著增加叶绿素a的含量,但照射天数延长后效果不明显。而T3(红光:蓝光=1∶3)光质在照射7 d时,能显著增加叶绿素b含量。T1(红光:蓝光=3∶1)光质照射7 d时、T2(红光:蓝光=1∶1)光质组照射21 d时能明显增加可溶性糖含量。T1(红光:蓝光=3∶1)光质照射14 d时、T3(红光:蓝光=3∶1)光质照射21 d时能明显增加可溶性蛋白质含量。同时实验发现 T2(红光:蓝光=1∶1)光质能明显降低MDA浓度,T3(红光:蓝光=1∶3)光质处理下脯氨酸含量有明显减少。表明光质处理可能也对叠鞘石斛造成了一定的胁迫,可能更有利于叠鞘石斛的生长。

Reference (20)

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return