Study on Tissue Culture and Rapid Propagation Technology of Polygonatum sibiricum from Sichuan Province

The tubers of Polygonatum sibiricum were selected as explants, and the pretreatment method of explants, the best induction, proliferation and rooting culture scheme were explored. The best tissue culture technology of Polygonatum sibiricum was analyzed and screened, and the tissue culture technology system of Polygonatum sibiricum was established. The results showed that the best pretreatment method of explants was to disinfect the surface of explants with 75% ethanol solution for 1 min, and then soak in 0.1% HgCl₂ solution for 15 min. The best induction medium was MS + 6-BA 5.0 mg·L⁻¹ + NAA 0.1 mg·L⁻¹. The best proliferation medium was MS + 6-BA 0.5 mg·L⁻¹ + NAA 0.05 mg·L⁻¹ + 1/3 (Mg^{2 +} + Fe^{3 +}) ↑+ 1/2 KH₂PO₄↑+ AC 1 g·L⁻¹. The best rooting medium was MS + 6-BA 0.05 mg·L⁻¹ + NAA 1.0 mg·L⁻¹ + AC 1 g·L⁻¹ 5 g·L⁻¹, and the rooting rate could reach more than 85%. The survival rate of rooting seedlings and non-rooting seedlings could reach more than 95% after outdoor acclimatization and transplanting.