Physiological and Biochemical Responses of Forest Tree Seedlings of 4 Species at the Early Growth Stage under Heavy Metal Cadmium (Cd) Stress
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摘要: 为研究4种乡土木本植物（五小叶槭Acer pentaphyllum、红椿Toona ciliata、桤木Alnus cremastogyne、云南松Pinus yunnanensis）幼苗生长对Cd胁迫的应对机制，本研究分析了这4种乡土木本植物幼苗在不同镉浓度（分别添加0~100mg·L-1氯化镉溶液）胁迫下的外部形态、超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）、谷胱甘肽（GSH）、丙二醛（MDA）和叶绿素（CHL）含量的变化。结果显示:外部形态方面，五小叶槭和桤木的地下部分长度显著下降，红椿地上部分显著下降，云南松地上和地下部分均无显著变化；SOD活性方面，五小叶槭、红椿、桤木和云南松总体均随Cd浓度的增加显著增加；CAT活性方面，五小叶槭、红椿和云南松总体上随Cd浓度的增加显著增加，桤木仅Cd浓度为100mg·kg-1时显著高于对照组；POD活性方面，五小叶槭、红椿、桤木和云南松总体上均随Cd浓度的增加显著增加；GSH方面，随着Cd浓度的增加，五小叶槭和云南松总体呈显著减少趋势，红椿和桤木略有减少；MDA和CHL含量方面，4种林木总体均表现为在高浓度Cd胁迫时显著下降，低浓度时则各有不同。总体上，在镉胁迫环境下这4种林木幼苗在生理生态方面发生了明显变化，并表现出较好的低浓度耐受性。Abstract: In order to study the response mechanism in the early stage of seedling growth under Cd stress,the changes of physiological and biochemical indexes were analyzed,which included external morphology,activities of superoxide dismutase (SOD),Peroxidase(POD) catalase (CAT),contents of glutathione (GSH),malondialdehyde (MDA) and chlorophyll (CHL) in 4 kinds of native woody plants(Acer pentaphyllum, Toona ciliata, Alnus cremastogyne, Pinus yunnanensis).The results were showed as below.In terms of external morphology,the underground length of Acer pentaphyllum and Alnus cremastogyne decreased significantly,and the upper part of T.ciliata dropped significantly,and there was no significant change for the ground and underground parts of P.yunnanensis.In SOD activity,Acer pentaphyllum, T.ciliata, Alnus cremastogyne and P.yunnanensis increased significantly with the increase of Cd concentration.As for CAT activity,Acer pentaphyllum, T.ciliata and P.yunnanensis increased significantly with the increase of Cd concentration,while Alnus cremastogyne was significantly higher than that of control group only when Cd concentration was 100 mg·kg-1.In POD activity,Acer pentaphyllum, T.ciliata, Alnus cremastogyne and P.yunnanensis increased significantly with the increase of Cd concentration.On the GSH side,with the increase of Cd concentration,the contents of Acer pentaphyllum and P.yunnanensis decreased significantly,and T.ciliata and Alnus cremastogyne were not significantly reduced.In terms of MDA and CHL content,these 4 kinds of forest trees showed a significant decrease in high concentration Cd stress,but varied in low concentration.In general,under the stress of cadmium,the seedlings of these 4 kinds of trees changed significantly in terms of physiology and ecology,and showed good tolerance in low concentration.
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Key words:
- Cadmium stress;
- Forest trees;
- Seedling;
- Antioxidant system
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