A Prelimiary Study of the Identification of Components of Sex Pheromone from Parocneria orienta Chao and its Trapping Effects in the Field
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摘要: 采取正己烷溶剂浸提法提取蜀柏毒蛾处女雌蛾性信息素腺体中的性信息素,运用气相色谱-触角电位联用仪(Gas Chromatography-electroantennographic Detection, GC-EAD)测定蜀柏毒蛾雄蛾触角对雌蛾性信息素腺体提取物中性信息素成分的活性反应,并运用气相色谱-质谱联用仪(Gas Chromatograph-Mass Spectrum, GC-MS)鉴定其活性反应成分。GC-EAD结果显示雄蛾触角对雌蛾性信息素腺体提取物中的2种成分有较好的反应。GC-MS分析结果表明能引起雄蛾触角电生理反应的成分为顺-9-十八碳烯醛和顺-9-十八碳烯醇。林间试验表明,顺-9-十八碳烯醛和顺-9-十八碳烯醇诱芯对蜀柏毒蛾有相近的诱集效果,表明顺-9-十八碳烯醛和顺-9-十八碳烯醇是蜀柏毒蛾性信息素主要成份。通过对复合组分配方顺-9-十八碳烯醛:顺-9-十八碳烯醇=1:9(200μg)、顺-9-十八碳烯醛:顺-9-十八碳烯醇=9:1(200μg)和单组分配方顺-9-十八碳烯醛(200μg)、顺-9-十八碳烯醇((200μg)4种配方诱芯林间诱集效果试验,结果4种配方均具有一定的诱集效果,但复合组分诱集效果比单组分诱集效果好。Abstract: The sex pheromone was extracted from sex pheromone glands of virgin female moths of P. orienta by n-hexane. The sensitive reaction of male moths antenna to the crude sex pheromon was detected by GC-EAD and the components of the sex gland extracts that consistently elicited antennal responses were identified by GC-MS. The results indicated that male moths antenna had sensitive reaction to two components of the extracts, which were Z-9-Octadacenal and Z-9-Octadacenol. In field trials, traps baited with either Z-9-Octadacenal or Z-9-Octadacenol alone caught a similar amount of male moths, which meant that Z-9-Octadacenal and Z-9-Octadacenol were the main components of the sex pheromone of Parocneria orienta. the combination of Z-9-Octadacenal and Z-9-Octadacenol in 1:9 or 1:9 ratio caught more males than single component traps.
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Keywords:
- Parocneria orienta /
- Sex pheromone /
- Component identification /
- Trapping
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期刊类型引用(4)
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