4株天牛致病菌对川硬皮肿腿蜂的毒力测定

陶园媛; 向茂榕; 刘昊泽; 王理顺; 何勇; 杨伟; 杨桦; 杨春平

doi:10.16779/j.cnki.1003-5508.2017.04.007

用微信扫码二维码

分享至好友和朋友圈

English

旧版入口

WE ARE COMMITTED TO REPORTING THE LATEST FORESTRY ACADEMIC ACHIEVEMENTS

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

4株天牛致病菌对川硬皮肿腿蜂的毒力测定

陶园媛 , 向茂榕 , 刘昊泽 , 王理顺 , 何勇 , 杨伟 , 杨桦 , 杨春平

文章导航 > 四川林业科技 > 2017 > 38(4): 30-33

陶园媛, 向茂榕, 刘昊泽, 王理顺, 何勇, 杨伟, 杨桦, 杨春平. 4株天牛致病菌对川硬皮肿腿蜂的毒力测定[J]. 四川林业科技, 2017, 38(4): 30-33. doi: 10.16779/j.cnki.1003-5508.2017.04.007

引用本文:

TAO Yuan-yuan, XIANG Mao-rong, LIU Hao-ze, WANG Li-shun, HE Yong, YANG Wei, YANG Hua, YANG Chun-ping. Virulence Determination of Four Monochamus alternatus Pathogens on Scleroderma sichuanensis[J]. Journal of Sichuan Forestry Science and Technology, 2017, 38(4): 30-33. doi: 10.16779/j.cnki.1003-5508.2017.04.007

Citation:

TAO Yuan-yuan, XIANG Mao-rong, LIU Hao-ze, WANG Li-shun, HE Yong, YANG Wei, YANG Hua, YANG Chun-ping. Virulence Determination of Four Monochamus alternatus Pathogens on Scleroderma sichuanensis[J]. Journal of Sichuan Forestry Science and Technology, 2017, 38(4): 30-33. doi: 10.16779/j.cnki.1003-5508.2017.04.007

4株天牛致病菌对川硬皮肿腿蜂的毒力测定

doi: 10.16779/j.cnki.1003-5508.2017.04.007

详细信息

四川农业大学林学院森林保护实验室，四川成都 611130

作者简介:
陶园媛,女,四川农业大学林学院森林保护专业2017届本科生

基金项目:
四川省大学生创新训练计划项目201410626023。
中图分类号: S763.3

Virulence Determination of Four Monochamus alternatus Pathogens on Scleroderma sichuanensis

More Information

Forest Conservation Laboratory of Forestry College, Sichuan Agricultural University, Chengdu 611130, Sichuan, China

摘要: 利用肿腿蜂携菌防治天牛,可发挥肿腿蜂主动搜索和病菌致病力强的优势,收到较单纯放蜂或喷施菌剂更好的效果。但需保证携带的病菌不会显著影响肿腿蜂的寄主搜索能力。为了利用川硬皮肿腿蜂携带病原菌防治松墨天牛,本文测定了4株松墨天牛致病菌的孢子粉和孢子悬浮剂对川硬皮肿腿蜂的毒力。结果表明:雌成蜂携带粉剂孢子的死亡率高于携带悬浮剂孢子的死亡率;当喷施孢子悬浮剂时,4个菌株的累计死亡率随浓度的增加而增加;白僵菌对川硬皮肿腿蜂的毒力较绿僵菌高,致死速率也较绿僵菌大。研究结果可为川硬皮肿腿蜂携菌防治松墨天牛的菌种选择和剂型研发提供依据。
- 川硬皮肿腿蜂;
- 松墨天牛;
- 白僵菌;
- 绿僵菌;
- 毒力测定
Abstract: Making use of Bethylidae carrying pathogens to prevent and control the longicorns could play advantage of active search of Bethylidae and strong pathogenicity of pathogens,better than releasing Bethylidae only or spraying microbial inoculum. However, it was necessary to ensure that the carrying pathogens would not significantly affect the Bethylidae' host search ability. In order to use Scleroderma sichuanensis carrying pathogens to prevent and control Monochamus alternatus,this paper measured the virulence of the conidial powder and spore suspensions of four strains of pathogens on S. sichuanensis. The results showed that the mortality rate of the powder spores in S. sichuanensis was higher than that of the suspension spores. When spraying spore suspension, the cumulative mortality of four strains increased along with the increasing concentration. For S. sichuanensis, the virulence of Beauveria bassiana was relatively higher than that of Metarhizium anisopliae, and the lethal rate of B. bassiana was also higher than that of M. anisopliae. Research results could provide some basis data for the selection of strains and development of microbial inoculum which was useful for S. sichuanensis carrying pathogens to prevent the M. alternatus.
- Scleroderma sichuanensis;
- Monochamus alternatus;
- Beauveria bassiana;
- Metarhizium anisopliae;
- Virulence determination

[1]	陈国全, 朱元伟, 张小国, 罗红, 李俊, 杨昌通, 朱鹏. 四川杉木2代种子园半同胞子代测定林生长性状的遗传变异分析 . 四川林业科技, 2024, 45(1): 66-74. doi: 10.12172/202305110001
[2]	刘培琴, 姜丽琼, 卢麒宇, 刘继, 谢永东, 浣杰, 李思言, 郑雯雪, 李文俊. 白及叶枯病病原菌分离鉴定与致病性测定 . 四川林业科技, 2024, 45(): 1-5. doi: 10.12172/202311140002
[3]	刘勇, 李祥乾, 李阳娣, 杨伟, 杨桦. 基于MaxEnt的松墨天牛在中国的潜在分布及其对气候变化的响应 . 四川林业科技, 2024, 45(2): 1-10. doi: 10.12172/202306120001
[4]	杨梅, 石艳, 石博文, 陈玲, 杨桦. 球孢白僵菌及酶增效剂对松墨天牛幼虫的毒力测定 . 四川林业科技, 2023, 44(4): 114-118. doi: 10.12172/202209230001
[5]	雷蕾, 李雨, 卢林, 杨墅冰, 杨桦. 利用川硬皮肿腿蜂携带高毒力白僵菌微胶囊防治松墨天牛的研究 . 四川林业科技, 2023, 44(2): 38-43. doi: 10.12172/202205240001
[6]	林明辉, 曾伟, 柯沛强. 薇甘菊叶斑病病原菌鉴定及寄主范围测定 . 四川林业科技, 2021, 42(2): 82-86. doi: 10.12172/202008050001
[7]	任金, 于正伦, 吴让伟, 李国耀, 陈小平, 李山琦, 杨兴伟, 付俊雪. 四川达州市松墨天牛生物学特性及防控措施最佳时机的研究 . 四川林业科技, 2021, 42(6): 46-53. doi: 10.12172/202108170002
[8]	梁勤彪, 麻文建, 马甲强, 李波, 王建平, 贺军花. 广元市松褐天牛危害情况调查 . 四川林业科技, 2020, 41(3): 104-107. doi: 10.12172/201911180002
[9]	吴桂康, 陈章铭, 杨桦, 杨伟. 云南松林松墨天牛发生规律及生物学特性 . 四川林业科技, 2019, 40(3): 82-86. doi: 10.16779/j.cnki.1003-5508.2019.03.017
[10]	马琴, 邵林娟, 杨桦. 川硬皮肿腿蜂学习经历对花椒与虎天牛虫粪挥发物的行为反应测定 . 四川林业科技, 2019, 40(4): 90-96. doi: 10.16779/j.cnki.1003-5508.2019.04.018
[11]	鲜伟, 吴开志, 陈保军, 陈国全. 四川杉木第二代无系种子园洪雅半同胞子代测定林生长分析 . 四川林业科技, 2018, 39(4): 45-48. doi: 10.16779/j.cnki.1003-5508.2018.04.011
[12]	杨莉, 周建华. 四川省松疱锈病菌的风险性分析 . 四川林业科技, 2017, 38(5): 76-78,104. doi: 10.16779/j.cnki.1003-5508.2017.05.018
[13]	魏永忠. 板栗花粉活力测定方法比较 . 四川林业科技, 2017, 38(3): 100-101. doi: 10.16779/j.cnki.1003-5508.2017.03.023
[14]	陈国熙, 马正发. 采用花绒寄甲防治松褐天牛的初步研究 . 四川林业科技, 2017, 38(1): 85-86. doi: 10.16779/j.cnki.1003-5508.2017.01.021
[15]	邱萍, 王敬. 飞机喷洒白僵菌防治马尾松毛虫预防性效果研究 . 四川林业科技, 2017, 38(3): 97-99. doi: 10.16779/j.cnki.1003-5508.2017.03.022
[16]	朱天辉, 彭娇洋, 刘倩, 贺敬宣, 马欣欣, 黄祖惠, 吴继云, 张霞. 莱氏野村菌复合粉炮防治杜仲梦尼夜蛾试验研究 . 四川林业科技, 2017, 38(4): 25-29. doi: 10.16779/j.cnki.1003-5508.2017.04.006
[17]	康小龙, 李晓冬, 李华清, 杨春琳, 康钉荣, 徐勇, 钟金, 王小梅, 马文俊. APF-1型诱捕剂防治“松褐天牛”试验研究 . 四川林业科技, 2016, 37(5): 41-44,92. doi: 10.16779/j.cnki.1003-5508.2016.05.009
[18]	姜勇, 贾廷彬, 何兴炳, 侯晓杰, 张楠, 王勇. 巨桉云斑天牛生物防治技术研究 . 四川林业科技, 2015, 36(4): 45-48. doi: 10.16779/j.cnki.1003-5508.2015.04.008
[19]	唐中芬, 华启尧, 杨佐忠, 王吉斌. 松诱木防治松墨天牛试验初探 . 四川林业科技, 2014, 35(3): 108-109. doi: 10.16779/j.cnki.1003-5508.2014.03.027
[20]	解锦华, 杨晓蓉, 曹霞, 胡刘平. 波美计快速测定尿素溶液浓度的方法探讨 . 四川林业科技, 2013, 34(2): 69-73. doi: 10.16779/j.cnki.1003-5508.2013.02.016

点击查看大图

计量

文章访问数: 351
HTML全文浏览量: 67
PDF下载量: 2
被引次数: 0

4株天牛致病菌对川硬皮肿腿蜂的毒力测定

doi: 10.16779/j.cnki.1003-5508.2017.04.007

四川农业大学林学院森林保护实验室，四川成都 611130

作者简介:
陶园媛,女,四川农业大学林学院森林保护专业2017届本科生

收稿日期: 2017-01-18

基金项目:

四川省大学生创新训练计划项目201410626023。

中图分类号: S763.3

关键词:

摘要: 利用肿腿蜂携菌防治天牛,可发挥肿腿蜂主动搜索和病菌致病力强的优势,收到较单纯放蜂或喷施菌剂更好的效果。但需保证携带的病菌不会显著影响肿腿蜂的寄主搜索能力。为了利用川硬皮肿腿蜂携带病原菌防治松墨天牛,本文测定了4株松墨天牛致病菌的孢子粉和孢子悬浮剂对川硬皮肿腿蜂的毒力。结果表明:雌成蜂携带粉剂孢子的死亡率高于携带悬浮剂孢子的死亡率;当喷施孢子悬浮剂时,4个菌株的累计死亡率随浓度的增加而增加;白僵菌对川硬皮肿腿蜂的毒力较绿僵菌高,致死速率也较绿僵菌大。研究结果可为川硬皮肿腿蜂携菌防治松墨天牛的菌种选择和剂型研发提供依据。

English Abstract

Virulence Determination of Four Monochamus alternatus Pathogens on Scleroderma sichuanensis

Forest Conservation Laboratory of Forestry College, Sichuan Agricultural University, Chengdu 611130, Sichuan, China

Received Date: 2017-01-18

Keywords:

Abstract: Making use of Bethylidae carrying pathogens to prevent and control the longicorns could play advantage of active search of Bethylidae and strong pathogenicity of pathogens,better than releasing Bethylidae only or spraying microbial inoculum. However, it was necessary to ensure that the carrying pathogens would not significantly affect the Bethylidae' host search ability. In order to use Scleroderma sichuanensis carrying pathogens to prevent and control Monochamus alternatus,this paper measured the virulence of the conidial powder and spore suspensions of four strains of pathogens on S. sichuanensis. The results showed that the mortality rate of the powder spores in S. sichuanensis was higher than that of the suspension spores. When spraying spore suspension, the cumulative mortality of four strains increased along with the increasing concentration. For S. sichuanensis, the virulence of Beauveria bassiana was relatively higher than that of Metarhizium anisopliae, and the lethal rate of B. bassiana was also higher than that of M. anisopliae. Research results could provide some basis data for the selection of strains and development of microbial inoculum which was useful for S. sichuanensis carrying pathogens to prevent the M. alternatus.