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2020 Vol. 41 Issue 2

2020 Vol. 41, No. 2 Catalogue

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A Study of Photosynthetic Characteristics and Leaf Traits of Salix cupularis with Different Ages in Alpine Sandy Land
DENG Dongzhou, LIU Cheng, HE Li, YAN Wuxian, CHEN Dechao, LI Jiayong
2020, 41(2): 1-7. doi: 10.12172/201908080001
Abstract:
Salix cupularis is the main plant for windbreak and sand fixation in northwest Sichuan. In this study, investigations were made on the differences of photosynthetic capacity and leaf traits among four age-class seedlings (1-, 2-, 8- and 11-year-old) of S. cupularis. The photosynthesis parameters and light response curves were measured using portable Li-6400 photosynthetic system (Licor, USA). The results showed that the diurnal variation of net photosynthetic rate (Pn) of S. cupularis showed an unimodal curve without midday depression. The Pn of 2-year-old S. cupularis was significantly higher than that of others, and the order was nursery 2-year-old > wild 11-year-old > nursery 1-year-old > nursery 8-year-old. The daily variation trend of Gs and Tr were the same, and the daily mean value of 2-year-old S. cupularis in nursery were higher than the other three age classes. The diurnal variation value of Ls showed the lowest in the morning, the Ls daily mean value of 2-year-old S. cupularis was lower than that of 1-year-old S. cupularis in nursery and 11-year-old S. cupularis in wild, and there was no significant difference compared with 8-year-old S. cupularis in nursery. WUE daily mean value of 11-year-old S. cupularis in wild was significantly higher than that of the other three age-class S. cupularis. Pn max of 2-year-old S. cupularis was significantly higher than that of others. LCP and Rd of 1-year-old S. cupularis were lower than that of the other three age-class S. cupularis. LCP and LSP of 11-year-old S. cupularis in wild were significantly higher than others in nursery. Leaf dry matter content of 11-year-old S. cupularis in wild was significantly higher than others in nursery. Leaf area, leaf dry weight, specific leaf area of 1-year-old S. cupularis in nursery and 11-year-old S. cupularis in wild were significantly lower than those of 2-year-old and 8-year-old S. cupularis in nursery.
Genetic Variation and Selection of Seedling Traits in Superior Eucalyptus cloeziana Families
CHEN Xiaozhong, ZHANG Linpin, CHEN Zhi, LIU Huanhuan, HUANG Zhen, LI Jiaman, YANG Hanbo
2020, 41(2): 8-14. doi: 10.12172/201912120001
Abstract:
In order to provide theoretical basis for genetic variation and selection of Eucalyptus cloeziana F. Muell. families at seedling stage, the growth (tree height and diameter at ground) of E. cloeziana families were investigated. One-year-old E. cloeziana was used to carry out genetic variation, variance, and genetic parameters estimation of seedling height and ground diameter, and superior families were comprehensively selected by the membership function method. The height and diameter at ground of E. cloeziana were significantly different among the families, and the repeatability was between 0.895 and 0.985. The coefficient of variation of height and diameter at ground were 44.01% and 65.96%, respectively. The family heredity and single heredity of height were 0.894 and 1.605, and the family and single heredity of diameter at ground were 0.985 and 2.935, respectively. Correlation analysis results showed that seedling height and ground diameter were negatively correlated, but not significant. The results of general combining ability analysis showed that the families with high general combining ability of different traits had great differences, which was difficult to carry out combined screening and further analysis was needed. There were abundant genetic variations in seedling growth traits of E. cloeziana families, which were under strong genetic control and had good genetic improvement potential. The height and ground diameter of E. cloeziana families were independent and could be individually oriented. Five optimal families (39, 19, 38, 25, and 13) were selected through the membership function method. Family 39 and 19 had extremely high genetic gain of ground diameter, and they could be used as excellent parents and (or) directive breeding materials.
A Study of Growth and Fruiting Characteristics of Idesia polycarpa Plantation in Hilly Area of Central Sichuan
DU Yangping, YAN Xianchun, LUO Jianxun, LIU Furong, WANG Haifeng
2020, 41(2): 15-21. doi: 10.12172/201912080001
Abstract:
Idesia polycarpa (IP) is an excellent native woody oil tree species in Sichuan. In order to provide scientific basis for the selection of superior family and fine clone in plantations in the future, studies were made of growth and fruit characteristics of Idesia polycarpa seed plantations. In this paper, the growth and fruit phenotypic characteristics of 5-year-old plantation in hilly areas of central Sichuan were investigated and statistically analyzed. The results showed that (1) the yield per plant varied from 2.59 kg to 26.75 kg, and the 100-grain weight of the fruit varied from 20.13 g to 63.92 g; (2) there were significant differences in growth traits and fruit phenotypic traits of the 25 primary selected plants. The results of correlation analysis between plant growth traits and fruit phenotypic traits showed that (a) there was a significant positive correlation among fruit sequence weight and fruit stalk thickness; (b) there was very significant positive correlation among tree height, DBH and crown width; (c) there was very significant positive correlation between the number of branches and the total number of branches; (d) there was significant positive correlation among the yield per plant and fruit inflorescence length, fruit inflorescence weight and net weight of each fruit inflorescence. In conclusion, there were significant differences in growth traits and fruiting characteristics between individual plants of Idesia polycarpa plantation, thus having great selection potential.
Analysis of Woody Plant Diversity in Four Universities of Nanchong City
XIAO Yajun, TANG Junfeng, ZHAO Xuzhe, Yan Xianchun
2020, 41(2): 22-28. doi: 10.12172/201912190002
Abstract:
The university campus is an important part of maintaining plant diversity of urban plant species. At present, relevant researches on campus plants mainly focus on landscape configuration, planning and design, while researches on plant community species diversity remains rare. In this paper, studies were made of the community structure characteristics and landscape structure characteristics of woody plants in universities campuses in Nanchong, including species composition, species diversity index, community similarity index, dominant species and their important values, plant ornamental characteristics and seasonal structure. The results showed that (1) there were 236 species of woody plants, belonging to 69 families and 144 genera; (2) the species were very rich in single campus, with Shannon-Wiener index ranging from 3.128 to 3.737, Simpson index ranging from 0.885 to 0.961, and Pielou index ranging from 0.702 to 0.842. However, the similarity of woody communities among different campuses was high, with Sørensen index between communities ranging from 0.466 to 0.654. In addition, it was found that the ornamental characteristics and seasonal structure of woody plants were similar in Nanchong universities, with relatively single dominant species and fewer rare and endangered species. This information has certain references for rational allocation of plant communities in campus gardens and optimization of campus environment, and provides reference for future research of scientific researchers.
A Geographical Visualization Method for Ecological Footprints——A Case Study of Hubei Province
ZHANG Jianhang, ZHOU Rongqi, ZHAO Yuxin
2020, 41(2): 29-34. doi: 10.12172/201912100002
Abstract:
Ecological footprints, as an important method and means to measure regional sustainable development, can quantitatively analyze and study the ecological profit and loss of the region. The current researches are mostly retrospective analysis or time series analysis and prediction, and few studies have been conducted on the geographical and spatial distribution of ecological footprints. With the development of remote sensing technology, abundant remote sensing products provide the possibility of studying ecological footprints at a more detailed scale. With the support of GIS, based on population density distribution data, noctilucent images, and MODIS products, this paper proposed a method to study ecological footprints at the kilometer grid scale, and also proposed two new methods to describe the spatial distribution of ecological footprints at the kilometer grid scale: "Output-type" ecological footprint and "Consumer-type" ecological footprint. Based on the estimation of the ecological footprint of Hubei province in 2010, analysis was made of the spatial pattern of the ecological footprint of Hubei province.
Diversity of Winter Waterbirds in Langzhong Section of the Middle Reaches of the Jialing River
YANG Wanjing, HU Jie, CHEN Hong, WEI Yong, LI Yanhong
2020, 41(2): 35-41. doi: 10.12172/202001190001
Abstract:
From January 2015 to January 2019, fixed line transects method was used to investigate the winter waterbirds diversity and abundance in Langzhong section of the middle reaches of the Jialing River. A total of 30 species of waterfowls was recorded, belonging to seven orders and nine families. Five Sichuan provincial key protected birds, including Gallinula chloropus, Phalacrocorax carbo, Podiceps cristatus, Podiceps nigricollis, Trachybaptus ruficollis, were recorded. Anas poecilorhyncha and Gallinula chloropus were dominant species of waterfowls in Langzhong section of the Jiangling River, accounting for 40.95% of the total number of observed waterfowls. This study showed that the waterbirds diversity in the line transects with more serious human disturbance was lower, indicating that human disturbance was the main reason for the lower birds diversity in this region.
The Current Status and Protection of Cercidiphyllum japonicum Resources in Sichuan Xuebaoding National Nature Reserve
PANG Dehong, ZHAO Ding, WU Yong, JIANG Zeyin, XIE Dajun, LIN Qiang
2020, 41(2): 42-46. doi: 10.12172/201912290001
Abstract:
Based on the literature search, interview, sample line and sample plot investigation, studies were made of the distribution, population structure and regeneration condition of Cercidiphyllum japonicum resources in Xuebaoding National Nature Reserve. The results showed that (1) there were 894 Cercidiphyllum japonicum plants in the Reserve, which were distributed in the elevation range of 1 600~2 400 meters; (2) there were 174 and 397 Cercidiphyllum japonicum plants in Mushuba and Dupingba respectively, accounting for 19.46% and 44.41% of the total; (3) there were 618 Cercidiphyllum japonicum plants with DBH between 5~45 cm, accounting for 69.13% of the total; (4) Cercidiphyllum japonicum trees were mainly distributed in the east, northeast, north, west and northwest slopes, accounting for 72.97% of the total.
A Study of the Photosynthetic Characteristics of Magnolia dawsoniana during Summer High Temperature Period in Chengdu
YAN Jinglin, LIU Guangli
2020, 41(2): 47-52. doi: 10.12172/201911270002
Abstract:
In order to explore the adaptability of introduced Magnolia dawsoniana during summer high temperature period in Chengdu, the photosynthetic indexes and chlorophyll content of four kinds of M. dawsoniana seedlings were measured under natural summer high temperature conditions in Chengdu Plain. The results showed that (1) the diurnal variation of net photosynthetic rate of the four kinds of M. dawsoniana seedlings was bimodal, with obvious midday depression of photosynthesis. The daily average net photosynthesis rate of grafed Magnolia dawsoniana seedlings was the largest. The diurnal variation of transpiration rate of the four kinds of seedlings was also bimodal, and the daily average transpiration rate of grafted Magnolia sieboldii seedlings was the largest. (2) The stomatal conductance of the four kinds of seedlings decreased obviously during the high noon temperature period, and after the high noon temperature period, the stomatal conductance of seedlings of Magnolia sieboldii showed the largest increase. Stomatal closure limited the photosynthetic rate during high temperature at noon, while in the evening, the decrease of light intensity made the photosynthetic rate decrease. (3) The correlation between the net photosynthetic rate of M. dawsoniana seedlings and environmental factors was highly consistent. (4) The order of chlorophyll content of the four kinds of seedlings was grafted Magnolia dawsoniana seedlings > Michelia alba seedlings > real seedlings > Magnolia liliiflora seedlings. Photosynthetic rate of four kinds of M. dawsoniana seedlings was inhibited under high temperature stress. The net photosynthetic rate and chlorophyll content of grafted Magnolia dawsoniana seedlings were the highest, and the adaptability was better, while the adaptability of real Magnolia dawsoniana seedlings was worse.
A Study of the Distribution Characteristics of Paeonia decomposita Resources in Sichuan
WU Huawei, ZHONG Jin, JIA Chen, LUO Jianxun
2020, 41(2): 53-57. doi: 10.12172/201911210001
Abstract:
In order to analyze the characteristics and stock of Paeonia decomposita resources in Sichuan, the current distribution patterns of Sichuan Paeonia decomposita resources were investigated. The results showed that Sichuan Paeonia decomposita were distributed in the Dadu River basin and the upper reaches of the Minjiang River in the Qionglai Mountain range and the Daxueshan Mountain range and they mainly grew on the northeast slope, southwest slope, occasionally on the north slope and east slope. They were often distributed in patches or scattered, and at an altitude of 1 946~2 988 m, the optimum altitudes were 2 200~2 600 m. They were often found in prickly shrubs such as wild pepper, Berberis chrysantha, witch hazel and so on, but rarely in forests. The soil was mainly mountainous yellow soil and mountainous brown soil, weakly acidic, loose and permeable, with good drainage. The parent rock was granite. In areas with small canopy density and high soil humidity, the natural regeneration ability was strong.
Analysis of Medicinal Plant Resources and Diversity Characteristics in Jiudingshan Nature Reserve of Sichuan Province
WANG Ge, TANG Yuansheng, YU Xiaogang, LIU Zhiwu, ZHENG Xiong, FU Kanghong, SHEN Deli, GU Yunjie
2020, 41(2): 58-63. doi: 10.12172/201912190001
Abstract:
The Jiudingshan Provincial Nature Reserve in Sichuan is located in one of the mountainous regions, which are the transition areas from the Qinghai-Tibet Plateau to the Sichuan Basin. The mountains are tall and climb from 700 m to nearly 5 000 m. The vertical habitat changes significantly, which breeds abundant medicinal plants resources. Totally 239 species of medicinal plants belonging to 201 genera and 95 families were found in Jiudingshan Nature Reserve. In term of species and genus diversity, there were more larger families (over 21 species), accounting for 26.32% of the total families. The middle families (6~10 species) were relatively fewer, accounting for 10.53% of the total families. The largest genera (over 21 species) had obvious advantages in quantity, accounting for 46.7% of the total number of genera. In term of the diversity of life forms, herbal medicinal plants (145 species) were dominated, accounting for 60.67% of the total species. Our study showed that Jiudingshan Nature Reserve was rich in species of medicinal plants, with various types of growth types and functions, which had great valuables for scientific research, protection and utilization.
Preliminary Study of Bougainvillea Greening Technology on Overpasses and Viaducts
CHEN Ziliang
2020, 41(2): 64-68. doi: 10.12172/201904230001
Abstract:
In this study, investigation was made on the current situation of three-dimensional greening of Bougainvillea on overpasses and viaducts in many cities in South China. The statistics and analysis were mainly carried out from the following aspects: substrate, pot containers, Bougainvillea varieties, steel structure design, irrigation and drainage equipment and the flowering period regulation of Bougainvillea. The purpose was to find the best greening technology aspects. Some suggestions were put forward for the future three-dimensional Bougainvillea greening on overpasses and viaducts in Southern cities in China.
Effects of Changlin Camellia oleifera Varieties on Yield and Quality in the Mountainous Areas of Suichang County
FAN Xiangzhen, ZHONG Zilong, YE Chenyu, XU Zhiheng, ZHOU Zhifeng
2020, 41(2): 69-73. doi: 10.12172/20192110001
Abstract:
In order to screen out the varieties of clonal Changlin Camellia oleifera suitable for planting in the mountainous areas of southwestern Zhejiang Province, seven new Camellia oleifera varieties such as Changlin No.3 and No.4 were used as the studied materials, and a complete randomized block design comparison experiment was used. The tree height, crown width and yield were studied after seven years of planting. The results showed that the average height of No.4, No.23, No.40 and No.166 was above 210 cm, and the average crown width of No.4, No.23, No.40, No.53 and No.166 reached 215 cm. In 2018, the average yield per plant of Changlin NO.3, NO.4, NO.23, NO.40, NO.53 and NO.55 reached above 4 kg, which was 81.61%~291.03% higher than the control. In 2019, the average yield per plants of Changlin NO.3 and NO.4,No.23, No.40, No.53 and No.55 reached above 3 kg, which increased by 30.34%~194.87% compared with the control. The dry seed yield of the seven varieties of Changlin species were 2.06%~18.87% higher than the common species. As for dry kernel yield of No.23, No.55 and No.166 was 1.73%~5.63% higher than that of common species, and the oil content of Changlin No.23 was 52.95%, which was 9.65% higher than that of the common species. According to the comprehensive analysis of various indicators of various varieties, Changlin No.4, No.23, No.40 and No.55 varieties had the characteristics of high yield, high quality, adaptability and resistance, and could be popularized as the main varieties in Suichang county.
Supplementary Description of Rhododendron feddei in Guizhou
FENG Bangxian, YANG Bing, LI He, Yuan Congjun, Long Haiyan, DAI Xiaoyong
2020, 41(2): 74-77. doi: 10.12172/201911260002
Abstract:
In this paper, the floral morphology and leaf size of Rhododendron feddei were described and supplemented. Inflorescence subapical, usually 5-flowered; calyx reduced to an undulate rim, glabrous; corolla funnelform 5, corolla tube cylindrical; tube ca. 1.2 cm, tapering narrow toward base; tamens 10, slender, exserted twice longer than corolla, filaments glabrous. This species was similar to Rhododendron stamineum, but it was easily distinguished due to the stiff bristles in midvein of the leaf back midrib. This paper provided some valuable information for further taxonomic revision of Rhododendron species.
Analysis and Countermeasures of Forest Resources in Lezhi County
XU Zhengjingru, LI Depeng, CHEN Jijun, YANG Xue, BAI Bin
2020, 41(2): 78-82. doi: 10.12172/201911270003
Abstract:
Based on the investigation of forest resources planning and design in Lezhi county in 2016, a description was made of the current situation and characteristics of forest resources in Lezhi county. Depending on the existing or possible problems in the current forest resources, this study put forward some countermeasures for scientific management, such as strengthening forest management and protection, implementing comprehensive forestry development, strengthening forest protection ability and improving the level of information establishment, in order to provide references for the high-quality development of forest resources in Lezhi County.
Effects of Capsule Seeds on Euphorbia pulcherrima Root Activity and Soil Moisture
CHEN Yonglan, XIE Cheng, YANG Yaojun, YU Jiaxin, FU Chun
2020, 41(2): 83-89. doi: 10.12172/201912260006
Abstract:
Soil fertility is not only an important part of soil quality, but also the foundation of land productivity. With the development of precision agriculture, the research on the relationship between soil fertility and plant root activity has become one of the hotspots in modern soil science. The purpose of this experiment was to investigate the effects of capsule seeds containing lye grass seeds and hyperabsorbent bamboo fiber microbial fertilizer on the texture and structure of different kinds of soil and the growth and development. Methylene blue method was used to measure the dynamic changes of root activity and soil moisture of lysophylline under the action mechanism of capsule seeds. The results showed that (1) capsule seeds had different effects on the water content of the three kinds of soil and the root activity of lysophylline; (2) capsule seeds could obviously increase the soil moisture content in sandy soil and loess. Under the same initial humidity, the soil moisture content of experimental group and control group was kept at the same level, reaching the highest on the 8th day. In the subsequent measurement, the soil moisture content of capsule seeds was significantly higher than that of the blank group, while the control effect of capsule seeds on soil moisture was not obvious under loam condition. (3) The root activity of lysophylline seeds was relatively improved when the three kinds of soil was treated with capsules. The results showed that capsule seeds played a certain role in controlling soil moisture and improving plant root activity.
Species Composition Analysis of Vascular Plants in Six Mountains in Haitan Island
BAI Peipei, WEI Kai, HE Yaqin, SHI Xiaojie, DENG Chuanyuan
2020, 41(2): 90-94. doi: 10.12172/201912020002
Abstract:
In order to find out the main plant resources in Haitan Island, a plant survey was carried out in 6 mountains of Haitan Island from May to September 2018. The results showed that (1) there were 267 species of vascular plants belonging to 221 genera in 87 families, including 35 tree speices, 41 shrub speices, 22 vines species, 169 herb speices, and 32 invasive alien species; (2) dominant families (more than 10 species) were Compositae, Gramineae and Leguminosae, with regional monotypic families and monotypic genera dominanted; (3) Herbs species dominated the growth Type. Leaf features were dominated by single leaf lobular type, leaf coat and non-whole leaf. The flower had a long life. In the fruit type, capsule, achene, stone fruit, and berries were dominant. The environment in Haitan Island was harsh, and the species of plants that could survive on the island were limited to some extent. Plant life types and leaf characteristics all indicated the adaptation of plants to the harsh island environment. The invasion of plants needs more attention.
Karyotype Analysis of Cyrtotrachelus buqueti Guer
WANG Zhenyu, WEI Ji, LIAO Hong, YANG Yaojun, LONG Wencong
2020, 41(2): 95-99. doi: 10.12172/201912210003
Abstract:
Taking the testis of male adults of Cyrtotrachelus buqueti Guer as the experimental materials, the chromosome number and morphology of C. buqueti were observed by acetic acid magenta tabletting method, and the karyotype characteristics were analyzed in this study. The results showed that the chromosome number of C. buqueti was 2n = 22, including 10 pairs of autosomes and 1 pair of sex chromosomes, all of which were centromeres of middle/sub middle, NF = 22. The sex determination mechanism of C. buqueti was Xyp type, and the karyotype formula was 2n = 22 = 16m + 4sm + Xyp. The percentage of chromosome with the longest chromosome to the shortest chromosome ratio greater than 4∶1, and the percentage of chromosome with the arm ratio greater than 2∶1 was 0.1. So the karyotype of C. buqueti was 2C type, and no secondary constriction and satellite characteristics were observed. The results supplemented the blank of the basic research on the classification characteristics of C. buqueti chromosome.
The Ultrastructure of Respiratory System of Cyrtotrachelus buqueti Guer
DENG Fuxiang, GUO Qing, LONG Wencong, YANG Haibo, YANG Yaojun
2020, 41(2): 100-107. doi: 10.12172/201912240002
Abstract:
In order to provide theoretical basis for the study of biological characteristics and systematic classification of Cyrtotrachelus buqueti Guer, the morphological anatomy, stereomicroscopy and scanning electron microscopy method were used to observe the morphological structure and characteristics of the respiratory system in the larva, pupa and adult stages of C. buqueti. The results showed that the larvae, pupae and adults of C. buqueti had developed integrated and strict four-stage tracheal system, and the inner wall of trachea was elastic and the spiral filaments formed a network structure which has the ability to resistant to stress. The adult had air sac and obvious spiracle structures. There were 9 pairs of spiracles on the adults’ body surface, 2 pairs of spiracles on the chest and 7 pairs of spiracles on the abdomen. Only one pair of spiracle on the middle chest was the external closed type, and the rest were the internal closed type. The spiracles were significantly smaller from the chest to the abdomen. The difference of respiratory system structure in the three stages was adapted to its living environment. The study was of great significance to the in-depth analysis of the relationship between the respiratory behavior, respiratory structure, growth, development and living habits of C. buqueti. It also enriched the morphological content of C. buqueti and provided theoretical basis for the systematic classification of weevil insects.
A Study of the Dynamic Changes of Volatiles on the Body Surface of Cyrtotrachelus buqueti Adults
LIU Liping, YANG Yaojun, LONG Wencong, LIAO Hong, FU Chun
2020, 41(2): 108-117. doi: 10.12172/201912260003
Abstract:
Body surface volatiles are important pheromones for individual communication among insects. Cyrtotrachelus buqueti, a pest in cluster bamboo forests, seriously limits the development of bamboo products and adversely affects the income of bamboo farmers in south of China. In this study, gas chromatography-mass spectrometry (GC-MS) technology and headspace sampling method were used to detect and analyze the volatile components on the surface of C. buqueti, providing scientific basis for controling the pest population. The results showed that a total of 28 compounds were detected inc 23 males and 23 females. GC-MS technology was also used to analyze the daily variation and ten-day variation of the volatiles on the body surface of C. buqueti. The results showed that (Z)-9-Tricosene and Heneicosane existed in the main volatiles of female insects throughout August, and the relative content of Heneicosane showed a downward trend from the first ten days to the second ten days, and the daily changes were basically the same, showing an increase first and then a decrease. Nonadecan, a male body surface volatile, existed in the whole August, and the relative content increased from the first ten days to the middle ten days and decreased in the last ten days. In summary, combined with the living habits of C. buqueti, it could be speculated that (Z)-9-Tricosene, Heneicosane and Nonadecan played a vital role in C. buqueti communication. These results also established a theoretical foundation for the development of C. buqueti pheromone.
Comparison of Cellulase Activity in Different Intestinal Segments of Cyrtotrachelus bugueti Adults
TANG Xiaojuan, YANG Yaojun, LUO Chaobing, LONG Wencong, FU Chun
2020, 41(2): 118-125. doi: 10.12172/201912260004
Abstract:
In order to compare and objectively evaluate the cellulase activity in different intestinal segments, the activity of three types of cellulase in three segments (foregut, midgut, hindgut) of Cyrtotrachelus bugueti adult were measured at different pH and temperatures. The results showed that (1) there was a complete enzyme system in the intestinal tract of C. bugueti adults; (2) the CX enzyme in the foregut of C. bugueti adults had a wider adaptive range of temperature and pH, and had greater stability; (3) the optimal temperature (T) was 50~70 ℃, and the optimal pH was 6~8. No enzyme activity was detected under high temperature and strong alkali conditions, and C1 enzyme in the foregut had the maximum enzyme activities of 0.0349 mol·Min−1·Mg−1 (pH=3, T=40 ℃) compared with the other two segments; (4) the adaptation range of C1 enzyme in the midgut was much larger than that of the other two segments. The optimum temperature was 30~70 ℃ and the optimum pH was 5~8. No enzyme activitiy was detected under strong acid and strong alkali conditions; (5) the optimum temperature range of the three enzymes in hindgut was 30~50 ℃, and the optimal pH range was 5~8, which was narrower than the optimal temperature and pH range of foregut and midgut of C. bugueti adults.
A Study of Cellulase Activity in Intestine Tract of Cyrtotrachelus bugueti Larvae
HE Hui, YE Yaojun, YANG Yaojun, FU Chun
2020, 41(2): 126-132. doi: 10.12172/202003090005
Abstract:
In order to explore the optimal pH value and temperature of the enzyme activity in the intestinal tract of Cyrtotrachelus buqueti larvae, different cellulase activities in different segments of intestinal tract (foregut, midgut, hindgut) were carried out under the interactions of different pH values and different temperatures. The optimum temperature range of exo-β-1,4-glucosidase activity was 50−60 °C (0.03 ± 0.005 μmol·min−1·mg−1), and the optimum pH was 5−6 (0.02 ± 0.005 μmol·min−1·mg−1). The optimum temperature range of endo-β-1,4-glucosidase activity was 50−60 °C (0.23 ± 0.005μmol·min−1·mg−1), and the optimum pH was 4−7 (0.02 ± 0.005 μmol·min−1·mg−1). The optimum temperature range of β-glucanase activity was 40−60 °C (0.02 ± 0.005 mol−1·mg−1), and the optimum pH was 4−7 (0.02 ± 0.05 μmol·min−1·mg−1). Temperature and pH had significant effects on cellulase activity in the insect intestinal tract.
Preliminary Investigation and Control Countermeasures of Scale Insects on Garden Plants in Panzhihua City
XIE Cuiping, ZHANG Haiyan, MA Shuyun, LI Tang, LIANG Li, LI Juan
2020, 41(2): 140-143. doi: 10.12172/201911260001
Abstract:
In order to find out the harm of scale insects on garden plants in Panzhihua city and to understand the species and occurrence regularity of scale insects, regular investigation and random sampling were conducted to investigate and study the scale insects in the built-up areas of Panzhihua City. The results showed that there were 16 species of scale insects in the garden plants in the built-up area of Panzhihua City, among them, Phenacoccus solenopsis Tinsley, Aulacaspis yasumatsui Takagi, Icerya purchase Maskell, Pseudococcus adonidum, Ceroplastes japonica Green were more harmful.